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September 3, 1938

VITAMIN B1METHODS OF ASSAY AND FOOD SOURCES

JAMA. 1938;111(10):927-934. doi:10.1001/jama.1938.72790360013008
Abstract

The importance of vitamin B1 in physiology and pathology has emphasized markedly the need of devising accurate methods for the quantitative determination of this substance in foods. Until recently the chemical identity of vitamin B1 was unknown, rendering it impossible to develop chemical methods of analysis, and accordingly recourse was had to biologic methods of assay using rats and pigeons as test animals. Much information regarding the vitamin B1 content of foods has been gained in this way, especially by the method in which the growth of rats was used as the measure of potency. This paper presents the technical aspects of various methods of bio-assay for vitamin B1 from the point of view of quantitative interpretation of the results obtained and discusses briefly the chemical methods that are being developed. It concludes with an evaluation of foods as sources of the vitamin.

BIOLOGIC METHODS WITH 

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