The rapid typing of pneumococci is becoming increasingly important with the development of therapeutic serums for the various types. The Neufeld method is admirably suited when the organisms are present in sputum, but difficulties are encountered when typing is attempted from cultures. The macroscopic agglutination of cultures requires the use of pipets and test tubes, and the quantity of serum required represents considerable expense. We have employed a microscopic precipitin technic by which the pneumococci can be typed from cultures quickly and with a very small amount of immune serum. The technic proposed by Brown1 for grouping the hemolytic streptococci has been adapted for this purpose.
PREPARATION OF THE ANTIGEN
Cultures of pneumococci from blood, spinal fluid or other sources taken during life or at autopsy may be grown in meat infusion broth containing a few drops of serum or ascitic fluid2 incubated at 37 C. for from
Schaub IG, Reid RD. A MICROSCOPIC PRECIPITIN TEST FOR TYPING PNEUMOCOCCI. JAMA. 1938;111(14):1285–1286. doi:10.1001/jama.1938.72790400002008a
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