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The second edition of this laboratory manual is but little changed from the first, which was a manual for poorly equipped laboratories, all the determinations being done with inexpensive apparatus. There is a colored plate of spectrums of hemoglobin and its derivatives. There seems to be some uncertainty as to what is meant by the spectrum of acid hematin, since it is insoluble in water, alcohol, ether or chloroform. Furthermore, on page 89 it is stated that acid hematin treated with ammonium sulfide will be altered to hemochromogen, and in the chart facing page 84 hemochromogen is said to contain denatured globin. Experience shows that it is necessary to add a protein to pure hematin to get it in solution. Therefore it seems more logical to consider acid hematin as oxidized hemachromogen and to reserve the name hemin for the protein free compound. Hemoglobin in blood is determined by changing
Practical Physiological Chemistry for Medical Students. JAMA. 1939;112(18):1859. doi:10.1001/jama.1939.02800180083039
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