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A daily analysis of the air for pollen is of great assistance to physicians treating allergy. The method of analysis commonly used consists of placing a greasy slide in the outside air for a period of twenty-four hours and counting the number of pollen granules deposited on a given unit of surface. Durham, who has done the most extensive work in this line, counts the number of granules deposited on 1.8 square centimeters of surface as a standard and assumes with Scheppegrell and others that this represents the pollen content of cubic yard of air. The area 1.8 square centimeters can be counted by crossing a slide five times from side to side with a low power microscope so adjusted as to cover a field 1.4 mm. wide.
This method of analysis is very useful because of simplicity. The limit of error, however, is gross and would make the method
Duke WW. A RAPID AND MORE ACCURATE METHOD OF DETERMINING THE POLLEN CONTENT OF THE AIR. JAMA. 1932;99(20):1686–1687. doi:10.1001/jama.1932.27410720004010d
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