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September 2, 1950


JAMA. 1950;144(1):47. doi:10.1001/jama.1950.02920010049011

In 1949 Mitchell and Hamilton1 reported that as much as 6.5 mg. of iron are lost daily in the sweat. This previously unsuspected avenue of iron loss was subjected to detailed analysis by Adams and his associates2 of the University of California at Los Angeles. With the method proposed by Leslie and Levin,3 sweat samples were obtained from a full length rubber glove drawn over one hand and forearm. All subjects were in a fasting state. Two glasses of water were drunk before the test. The other arm was placed in a water bath at 110 F. during the one hour sweating period. An average of about 10 cc. of opalescent sweat was collected from each normal person. Chemical analysis showed that this "cell-rich" sweat usually contained 6 to 10 micrograms of iron per cubic centimeter. Duplicate samples were rendered "cell poor" by centrifugation. Analysis showed that