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This monograph was written in Moscow, Russia, in 1938-1939; most of the early work was done with the help of Russian assistants. As the title indicates, the present volume deals with microscopic study of living tissues in contrast to the classical method of fixing, staining and mounting thin sections of dead tissues, to which the author alludes in his introductory chapter. Throughout the monograph the author points out that he and his collaborators studied microscopically only living organs "in vivo et in situ."
The material for study consisted chiefly of anesthetized frogs, rats and rabbits, as well as plants. The method of study with high-powered, specially constructed microscopes allowed even the use of oil immersion lenses on otherwise opaque organs whose blood supply was intact. The details of these instruments as well as the micromanipulators (used over twenty years) will be described in a separate volume.
The organs and tissues
Lebendige Gewebelehre: Eine Histophysiologie auf neuer Grundlage. JAMA. 1947;135(8):541. doi:10.1001/jama.1947.02890080071028
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