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September 11, 1948


JAMA. 1948;138(2):133. doi:10.1001/jama.1948.02900020029012

Numerous investigators have shown that it is possible to couple chemical agents with an antibody without destroying its specific activity. Marrack1 found that typhoid bacilli are specifically agglutinated by a typhoid antiserum prepared by coupling a red dye with the specific agglutinin. The bacilli thus agglutinated were colored red. Coons2 and associates of Harvard University prepared a tagged type 3 antipneumococcus serum by coupling it with a fluorescing substance. The coupled serum showed a blue fluorescence when irradiated with ultraviolet light. Clumps of type 3 pneumococci specifically agglutinated by this tagged serum also showed a blue fluorescence. They afterward3 prepared a tagged antiserum which fluoresced green. With this they were able to locate regions of high concentration of type 3 pneumococci in tissues of infected mice. On ultraviolet irradiation the fixed tissues in these areas fluoresced green. McClintock and Friedman4 combined uranium or malachite green with