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April 14, 1956

BLOOD VOLUME DETERMINATIONS WITH RADIOACTIVE CHROMIUM (Cr51) LABELED ERYTHROCYTESFEASIBILITY OF ROUTINE TOTAL RED BLOOD CELL VOLUME DETERMINATIONS IN A GENERAL HOSPITAL

JAMA. 1956;160(15):1312-1315. doi:10.1001/jama.1956.02960500042011b
Abstract

With the introduction of Na2Cr51O4 as a label for red blood cells and the observation that ascorbic acid prevents further uptake of radiochromium (Cr51) by erythrocytes, a plan suggested itself whereby routine total red blood cell volume determinations could be performed in a small general hospital.

Material and Methods

Each morning 50 ml. of fresh (24-48 hours old) group O Rh-negative blood, preserved in acid-citrate-dextrose mixture (this is enough for four patients), was withdrawn from the hospital blood bank and incubated with 250 p.c. of Na2Cr5104 for one hour at 37 C, with shaking at infrequent intervals. At the end of this period 10 ml. of sterile isotonic sodium chloride solution and 50 mg. of ascorbic acid was added and the mixture gently rotated for five minutes. Hematocrit determinations were made in triplicate with the International capillary hematocrit centrifuge. Ten milliliters of labeled blood was diluted with water to 1,000

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