In 1950, one of us (A. J. B.) observed that subcutaneous injections of hyaluronidase (wydase®), mixed with isotonic sodium chloride solution, resulted in clearing of urine in patients who previously had turbid urine with much sedimentation. The effect of hyaluronidase was manifest within 30 minutes after injection and persisted for 24 to 72 hours.1 In attempting to establish a mechanism by which hyaluronidase produces this effect, we examined urines under a microscope equipped with a standard dark-field condenser to ascertain whether hyaluronidase caused a change in the colloid chemical condition of the urine under investigation. Preliminary observations appeared to establish a correlation between the injection of hyaluronidase and the number of colloidal particles discernible in the urine sample. Certain colloidal phenomena (e. g., adhesion) made the statistical validity of this method questionable. In addition, considerable dispute as to the significance of such examination is already apparent. Since that time,
Butt AJ, Hauser EA, Seifter J. EFFECT OF HYALURONIDASE ON URINE AND ITS POSSIBLE SIGNIFICANCE IN RENAL LITHIASIS. JAMA. 1952;150(11):1096–1098. doi:10.1001/jama.1952.03680110036011
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