A tablet test for albuminuria, requiring but one drop of urine, has been studied in the department of pathology of Harper Hospital. The test is based on the effect of protein on a pH indicator, a phenomenon first described by Sorensen1 in 1909. This effect, the so-called protein error of indicators, has long been an obstacle to biochemists attempting to use indicators to measure the pH of body fluids. It was later adapted to qualitative tests for proteins by Feigl and Anger2 and by Ishidate and Sakaguchi,3 The latter authors, Joukovsky and Vandervelden,4 and Ketomaa and Ruosteenoja5 studied large numbers of pH indicators, finally selecting the potassium salt of tetrabromophenolphthalein as most desirable for serum protein measurements. Exton6 devised a colorimetric test for albuminuria in 1925 with use of sulfosalicylic acid and bromphenol blue. He noted that bromphenol blue apparently stabilizes the sulfosalicylic-protein precipitate
Knights EM, Jablokow V. A ONE-DROP METHOD FOR DETECTION OF ALBUMINURIA. JAMA. 1957;165(10):1277–1279. doi:10.1001/jama.1957.72980280004011b
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