Microscopic examination of the urine is a relatively simple procedure and, when it is performed promptly and properly, can be very informative. In urinary tract infections the physician may depend upon the finding of pus cells and micro-organisms in the urine to aid him in making a diagnosis. Many methods of collecting, examining, and reporting these specimens have been recorded, but it has been our experience that unless the routine outlined is simple and definite, there will be so many sources of contamination that the results may be of little or no value. For example, a urine which has been collected at 6 a. m. and then allowed to stand at room temperature until 11 a. m. or later may show a large number and variety of micro-organisms, even if only a few contaminants were present in the fresh specimen. Hemolysis of red blood cells in urine allowed to stand
Doolittle LH, Cohen JR. RAPID METHOD OF DETERMINING URINARY INFECTION. JAMA. 1957;165(11):1391–1392. doi:10.1001/jama.1957.72980290001007
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