Tests which measure the time required for varying concentrations of test substances to completely immobilize all spermatozoa in a given volume of spermatic fluid have been used in evaluating the relative spermatocidal activity of these substances.1 Such dilutions are useful for measuring the intrinsic potency of an active ingredient in terms of minimal spermatocidal concentration, but the degree of homogenization required to provide reproducible results has no counterpart in coitus, and the relatively high values obtained are not applicable to defining the spermatocidal activity of contraceptive gels under conditions of use.
In order to approach normal conditions Gamble2 devised a "diffusion test" for evaluating the spermatocidal activity of undiluted contraceptive products. The Gamble procedure utilizes glass tubing 2 cm. in length with an internal diameter of 2 mm. in which a column of semen is brought into contact with a column of undiluted contraceptive jelly, cream, or suppository
Berberian DA, Slighter RG. CYTOMETER CHAMBER SPERMATOCIDAL TEST: AN IMPROVED TECHNIQUE FOR EVALUATING SPERMATOCIDAL ACTIVITY. JAMA. 1958;168(17):2257–2259. doi:10.1001/jama.1958.63000170001012
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