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April 25, 1959


Author Affiliations

Savannah, Ga.

From the Communicable Disease Center, Bureau of State Services, Public Health Service, U. S. Department of Health, Education, and Welfare, Technical Development Laboratories (Mr. Wolf and Dr. Harris) and the Public Health Service Hospital, Bureau of Medical Services, Public Health Service, U. S. Department of Health, Education, and Welfare (Dr. Dyer).

JAMA. 1959;169(17):1983-1987. doi:10.1001/jama.1959.03000340015004

Air-sampling for bacteriological studies was carried out in an operating room for nine days, during which 27 patients were operated on. The 34 colonies of Staphylococcus aureus so obtained were studied as to bacteriophage type. The most frequent type was 80/81. The colonies differed as to sensitiveness to most of the eight antibiotics tested, but all colonies were sensitive to polymyxin B. Eight of the colonies proved sensitive to every one of the antibiotics tested, but there was one colony, not typable, that was resistant to chlortetracycline, chloramphenicol, erythromycin, penicillin, oxytetracycline, and tetracycline. Nontypable colonies were obtained from the nares of two persons working in the operating room. One postoperative infection occurred; the organism concerned was a staphylococcus of type 80/81.