Since Papanicolaou1 first described a procedure of preparing smears for cytological examination numerous investigators have attempted to improve the method of fixation. Their principal purpose was to achieve good preservation of cells with a minimum time spent in fixing the slides and a convenient and safe means of transporting these slides to the laboratory.
The original method consisted of placing freshly prepared smears into a bottle containing a mixture of equal parts of alcohol and ether. The cellular fixation resulting from this method is excellent, and it is still being widely used even though the bottles are bulky, subject to breakage, and not suitable for mailing. Several workers have made innovations principally in order to render the slides amenable to transportation in a dry state.
Ayre and Dakin2 suggested fixing slides in alcohol-ether and then covering them with glycerine and another clean slide. Sagi and Mackenzie3 described
Ehrenreich T, Kerpe S. NEW, RAPID METHOD OF OBTAINING DRY, FIXED CYTOLOGICAL SMEARS. JAMA. 1959;170(10):1176–1177. doi:10.1001/jama.1959.63010100002012a
Coronavirus Resource Center
Customize your JAMA Network experience by selecting one or more topics from the list below.
Create a personal account or sign in to: