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December 2, 1961

Effect of Poliomyelitis Virus on Phospholipid Metabolism of HeLa Cell

JAMA. 1961;178(9):912-914. doi:10.1001/jama.1961.73040480001008

INCREASED PHOSPHOLIPID SYNTHESIS has been observed to occur, associated with a number of viral infections: skin of rabbits infected with Shope virus,1 chicken skin infected with fowlpox,2,3 mouse brain as a consequence of infection with Theiler's G D 7 virus,4 and KB cells with adenovirus.5 The uptake of radiophosphorus (P32) into cellular phosphorus fractions (inorganic, phospholipid, and nucleic acid) of the HeLa cell (a strain of malignant human epithelial cells maintained in vitro by serial cultivation since February, 19516) with and without the poliomyelitis virus has been investigated.7 This was the first report in which radiophosphorus was used to tag the poliomyelitis virus. This was confirmed by Maassab et al.8 and Contreras et al.9 Goldfine et al.10 have studied the incorporation of C14-nucleosides into HeLa cell ribonucleic acids and deoxyribonucleic acids during poliomyelitis virus infection.

The time course