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July 17, 1967

The Autohemolysis Test

Author Affiliations

From the Division of Hematology, Department of Medicine, and Laboratories, Long Island Jewish Hospital, New Hyde Park, NY, and Albert Einstein College of Medicine, of Yeshiva University, New York. Dr. Prager is now at Allentown (Pa) General Hospital.

JAMA. 1967;201(3):189-191. doi:10.1001/jama.1967.03130030059014

The purpose of this communication is not to present any original material, but to bring further attention to a laboratory screening procedure that is easy to perform and which yields useful information in the study of patients with hemolytic anemia. It is recognized that the nonspherocytic congenital hemolytic anemias are composed of a variety of syndromes with similar red blood cell (RBC) morphology. In 1954, the autohemolysis test was described,1 and helped to categorize the nonspherocytic congenital hemolytic anemias. The precise method of performing the autohemolysis test is described elsewhere.2 Briefly, the patient's blood is collected in a sterile manner and defibrinated. Two milliliters of the sterile defibrinated blood is added to sterile screw-capped vials. Sterility is important, for bacterial contamination will hemolyze RBC. Glucose is added to one vial and to another, adenosine triphosphate is added. One vial of the sterile defibrinated blood has no additive. All three