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March 11, 1968

A Simple and Reliable Test for Sickling

JAMA. 1968;203(11):990. doi:10.1001/jama.1968.03140110082028

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To the Editor:—  The paper of Schneider, Alperin, and Lehmann (202:419, 1967) pointed out the occasional difficulties and pitfalls of the standard sickling test performed in the clinical laboratory. A much simpler and more reliable test for the clinical laboratory is the slideelution test which we developed a few years ago (Blood24:198, 1964). This test is based on the fact that hemoglobin S is insoluble in high molar phosphate buffer. Blood smears are prepared from capillary blood in the same way as for staining, but the unstained slides are placed into Coplin jars filled with 50 ml of 2.4 O M phosphate buffer (20.9 g/100 cc of K2HPO4 and 16.3 g/100 cc of KH2PO4; the solution is stable and we have used it for longer than three months after preparation). Just prior to the placement of the slide a small amount