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November 11, 1968


JAMA. 1968;206(7):1425-1444. doi:10.1001/jama.1968.03150070007003

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New Blood Culture Method Developed 

Organisms Seen When Broth Fails  A new blood culture technique, which halves the time required for the standard broth culture, has been developed by New Jersey investigators.With this technique, culture inhibitors which might be in the blood are diluted out and red blood cells are removed before the sample is filtered and cultured.Preliminary results with this "dilution-filtration" technique were reported to the joint meeting of the American Society of Clinical Pathologists and the College of American Pathologists.The developers, a team from the Institute of Laboratory Medicine, Perth Amboy General Hospital, Perth Amboy, NJ, said the technique has won ready acceptance by the hospital staff.Their laboratory can grow and identify organisms in most instances within 24 hours, half the time needed for the broth technique.Dilution-filtration also recovers organisms that are missed by other techniques. It works even when a pa