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June 27, 1977

Re: Rapid Method for Tzanck Preparations

JAMA. 1977;237(26):2814. doi:10.1001/jama.1977.03270530022013

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To the Editor.—  In answer to some recent questions and for those interested in our staining method (237: 1119, 1977), I would like to add that prior fixation is not always necessary. If the specimen is prepared as previously outlined, it may be stained immediately (prior to drying) with the Paragon Multiple Stain; the results from this method are quite adequate, since the stain itself serves as a fixative. This is, in fact, how frozen sections are stained. It must be emphasized that this necessitates a staining facility almost at bedside. Therefore, although we do stain fresh material, we have found it technically easier to fix the tissue first.