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September 19, 1977

Bacterial Meningitis Diagnosis by Counterimmunoelectrophoresis

Author Affiliations

Faculté de Médecine Dakar, Senegal

JAMA. 1977;238(12):1248-1249. doi:10.1001/jama.1977.03280130030008

To the Editor.—  Cerebrospinal fluids (CSF) of 80 patients with purulent meningitis were studied, immediately after collection or storage at —20 °C, by counterimmunoelectrophoresis (CIE) for the presence of meningococcal, pneumococcal, and Haemophilus influenzae antigens. The results obtained by CIE were compared with the results of Gram-stained smears together with bacterial cultures.Haemophilus influenzae b anti-serum is produced in our own laboratory, using ATCC 10.211 strain and monospecific antiserum. Haemophilus influenzae a, c, d, e, and f were obtained from Wellcome laboratory. Omniserum containing antibodies against 83 specific types of pneumococcals and antiserum pools (A-I) were furnished by Statens Serum Institut of Copenhagen (Denmark). Meningococcal antiserum (A-B-C) were obtained from Pasteur Institute of Paris.The CIE was carried out in indubiose 1%, veronal/veronal-sodium buffer (pH 8.8). Sensitivity of CIE was tested with purified bacterial polysaccharides from pneumococcus type 1-2-3, H influenzae b ATCC 10.211, and meningococcus A vaccine (of Merieux