A colonic villous adenoma removed from a glucose-6-phosphate dehydrogenase (G6PD) heterozygous patient was studied. The mucosal cells were separated to eliminate the contaminating nonadenoma cells by enzymatic disaggregation followed by isokinetic gradient centrifugation. The mucosal cells from this colonic polyp contained a single G6PD isoenzyme. Our study demonstrates clonality of this type of polyp and the value of the cell separation techniques in the experimental oncology.
Adolphson CC, Prchal JT, Carroll AJ. Colonic Villous AdenomaMonoclonal Origin. JAMA. 1982;247(6):829–830. doi:10.1001/jama.1982.03320310077042
Customize your JAMA Network experience by selecting one or more topics from the list below.