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April 11, 1885


JAMA. 1885;IV(15):410-411. doi:10.1001/jama.1885.02390900018010

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Bacterium Culture.  —I have just received the copy of the Journal containing my letters about "Bacteria Culture," and with your permission would like to add a little to what I wrote then.In saying that agar agar is prepared like gelatine, it should be mentioned that the percentage of agar agar is much less, only one to three per cent. The dilution method (Verdünnungsmethod) of making a plate culture is especially advantageous, in that the quantity of material used as seed can be more easily controlled. For this, in addition to the plate ap paratus, there are necessary the usual platinum wire, which has a glass rod handle and which has its end bent into a very small circle, and three of the test tubes filled with gelatine, as before described, each containing one or two drachms (4-8 cc.) of gelatine solution. The gelatine having been

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