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In some investigations on this subject, the full records of which are published in the Zeitschrift für Hygiene, Bd. 2, 1887, D. Biondi took sputa from 50 persons, some in health, others ill, and injected it into animals. When pathogenic results followed the tissues were searched for microörganisms, and these cultivated and isolated. In this way he found 5 different kinds of microorganisms. Of these the Bacillus salivarius septicus was found in 20 per cent. of the sputa examined, and it was most abundant in the sputa secreted before mid-day. Dogs and mice into which.5-1 cc. of such sputum was injected died in the course of 24 to 72 hours. Before death there was usually fever, sometimes also continuous coma. In some cases death did not take place till after the lapse of 30 days, the animals gradually emaciating, and the hair falling, etc. After death œdema and hæmorrhage were
PATHOGENIC MICROORGANISMS IN SPUTA.. JAMA. 1887;IX(10):308–309. doi:10.1001/jama.1887.02400090020005
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