Since making my report on the investigations concerning the nature of tyrotoxicon, (report of Proceedings of the Michigan State Board of Health, October, 1886), I have continued my work, aided greatly by Messrs. F. G. Novy and E. V. Riker. We soon ascertained that if some butyric acid ferment be prepared as is ordinarily done in the preparation of butyric acid, and some of this be added to normal milk, and the whole be kept in closely stoppered bottles for eight or ten days, the poison will be developed in the milk in considerable quantity. The milk should be filtered, the filtrate neutralized with sodium carbonate, and then extracted with ether.
Having a strong solution of the poison in absolute alcohol, which had been obtained from milk inoculated as stated above, I added to it some platinum chloride and began to evaporate on the water-bath. As soon as the alcohol
VAUGHAN VC. THE CHEMISTRY OF TYROTOXICON: Its Action upon Lower Animals; and its Reflation to the Summer Diarrhoeas of Infancy.Read in the Section on State Medicine, at the Thirty-Eighth Annual Meeting of the American Medical Association, June, 1887. JAMA. 1887;IX(12):361–366. doi:10.1001/jama.1887.02400110009001b
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