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The author reported in detail a long series of experiments which are still in progress, and announced the results already obtained. Upwards of fifty inoculations have already been made. Two methods of inoculation have been employed—intra cranial inoculations after the method of Pasteur in the case of rabies, and subcutaneous or inter-muscular injections by means of hypodermic syringes. The inoculations and injections were made with aseptic precautions, and with sterilized instruments. In none of the experiments was there any sign of accidental infection, such as suppuration, etc. The material used for inoculation was in general obtained from the medulla or the spinal cord, and by culture in neutral or slightly alkaline flesh-glycerine-agar as recommended by Roux for the culture of tubercle bacilli. The tetanus material was taken, under aseptic precautions, from a horse and a mule dead of traumatic tetanus in the Veterinary Department of the University of Pennsylvania, the
SHAKESPEARE EO. ABSTRACT OF A PRELIMINARY REPORT OF EXPERIMENTAL RESEARCHES CONCERNING THE INFECTIOUS NATURE OF TRAUMATIC TETANUS.Read in the Section of Pathology of the Ninth International Medical Congress, September 6, 1887.. JAMA. 1887;IX(16):481–482. doi:10.1001/jama.1887.02400150001001
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