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Any process of hardening tissues rapidly at once attracts attention. Within recent years the freezing methods have been very much in use. Their rapidity and ease of application recommend them to the physician and pathologist alike, and the slight distortion occasioned by their use is more than compensated by constant results when properly employed. The fact that no prolonged fixing, dehydrating or constant changing of solutions is necessary, has long been the most potent argument for their use. Frequently, the chemical agent selected for hardening penetrates only the periphery, while this method insures a thorough and uniform hardening of the specimen. Large pieces are no bar to its use—they merely take more time to harden thoroughly while small pieces are more easily manipulated and less liable to tear.
There are many who use the sliding type of microtome exclusively, and must rely entirely on the ether method for their frozen
REHFUSS ME. A NEW EFFICIENT AND INEXPENSIVE FREEZING ATTACHMENT FOR THE SLIDING MICROTOME.. JAMA. 1908;L(16):1266–1267. doi:10.1001/jama.1908.25310420034002c
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