On July 23, 1907, Professor Chantemesse1 announced at a meeting of the Academy of Medicine in Paris a new characteristic reaction for the diagnosis of typhoid fever. Two months later2 he published the results of this diagnostic test applied to 128 patients, together with a brief description of its method of application.
The test consisted of the instillation into the conjunctival sac of one drop of a solution made as follows: Gelatin plates covered with an 18 to 20 hour old culture of virulent typhoid bacilli were washed with 4 to 5 c.c. of sterile water. The suspension thus obtained was heated to 60 C., centrifugated and the supernatant fluid withdrawn. The centrifugated organisms were then dried and triturated. A second suspension of these broken up bacillary bodies was then made and allowed to stand for from two to three days at 60 C. The extract thus obtained
HAMBURGER WW. THE OCULAR TYPHOID REACTION.PRELIMINARY REPORT OF A MODIFICATION OF THE OCULAR TEST OF CHANTEMESSE.. JAMA. 1908;L(17):1344–1345. doi:10.1001/jama.1908.25310430028001e
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