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Article
January 23, 1909

A SIMPLE METHOD FOR REMARKING THE SQUARES IN THE COUNTING-CHAMBER OF A ZEISS HEMATOCYTOMETER.

Author Affiliations

CAPE COLONY, SOUTH AFRICA

JAMA. 1909;LII(4):297. doi:10.1001/jama.1909.25420300037002c
Abstract

The inexperienced blood counter sometimes has difficulty in cleaning the slide and counting-chamber of the Zeiss hematocytometer and often resorts to the use of alcohol and ether with disastrous results. These agents remove the color from the demarcating lines of the various squares and the instrument is rendered useless. Some laboratory authorities have not been able to suggest anything better than rubbing the cell with a soft lead pencil and polishing with a soft linen cloth, or they advise the return of the slide to the maker for the purpose of remarking. Having noticed that the glass beads in the mixing-chamber had a strong affinity for Toison's fluid,1 I determined to rub a piece of gauze moistened in this solution gently over the counting-chamber. The result was excellent, the boundaries of the squares being clearly defined, and, moreover, they retain the stain far better than that given by the

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