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Article
October 30, 1909

A METHOD FOR COUNTING BACTERIA IN THE BLOOD

Author Affiliations

LAS VEGAS, N. M.

JAMA. 1909;LIII(18):1487-1488. doi:10.1001/jama.1909.92550180039002h

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Abstract

The pipette, in use for the leucocyte count with the Thoma-Zeiss hematocytometer or its modifications, is filled in the usual manner, blood being drawn to the 0.5 mark and the melangeur filled to 11. For the dilution we use a solution of 2 per cent. sodium citrate and 1 per cent. of sodium chlorid in water. Of this diluted blood, 1 milligram is measured with an accurate hematocrit scale or a specially marked, fine pipette. This milligram is distributed over a warmed slide in fine drops deposited in a row. About eight droplets usually will use up this quantity of dilution.

It is of the greatest importance to make the droplets as small as possible. The drop-films are allowed to dry well. They are next fixed with a mixture of 3 per cent. acetic acid in alcohol. No heat must be applied. When fixed

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