This article is only available in the PDF format. Download the PDF to view the article, as well as its associated figures and tables.
The pipette, in use for the leucocyte count with the Thoma-Zeiss hematocytometer or its modifications, is filled in the usual manner, blood being drawn to the 0.5 mark and the melangeur filled to 11. For the dilution we use a solution of 2 per cent. sodium citrate and 1 per cent. of sodium chlorid in water. Of this diluted blood, 1 milligram is measured with an accurate hematocrit scale or a specially marked, fine pipette. This milligram is distributed over a warmed slide in fine drops deposited in a row. About eight droplets usually will use up this quantity of dilution.
It is of the greatest importance to make the droplets as small as possible. The drop-films are allowed to dry well. They are next fixed with a mixture of 3 per cent. acetic acid in alcohol. No heat must be applied. When fixed
HOAG HJ, FEST FTB. A METHOD FOR COUNTING BACTERIA IN THE BLOOD. JAMA. 1909;LIII(18):1487–1488. doi:10.1001/jama.1909.92550180039002h
Customize your JAMA Network experience by selecting one or more topics from the list below.
Create a personal account or sign in to: