Since Schaudinn demonstrated the presence in syphilitic lesions of a spirillum which he called Spirochæta pallida, afterward changing the name to Treponema pallidum because the name Spirochæta pallida had already been given to another organism, numerous observers have attempted to utilize various methods in the hope of rendering the demonstration of the organisms more easy. Schaudinn made use of Giemsa's azur eosin polychrome mixture by which the organism is demonstrated as a pale slightly reddish-blue spiral. The various modifications of the Jenner or Romanowski stain have shown themselves of value, especially those of Leishman and Wright. The shortening, too, of the time necessary for the staining by Giemsa's method have increased the usefulness of this procedure although, perhaps, a less satisfactory stain results than when the original stain is used.
The identification of the organism, however, by all of these methods is far from easy and decidedly tiring
GURD FB. NEWER METHODS OF DEMONSTRATING THE TREPONEMA PALLIDUM WITH ESPECIAL REFERENCE TO THE INDIA-INK METHOD. JAMA. 1910;LIV(22):1779–1780. doi:10.1001/jama.1910.92550480001001g
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