One of the most important of the regressive staining methods used for general histological work is the eosinmethylene-blue method, hitherto only applicable to tissues fixed in Zenker's fixative. A successful stain by this method yields better results for cell-differentiation and the study of fine structures than any other single staining method. Its use has been limited because of the difficulty of securing constant results. Inquiries made in various laboratories, and the results of personal experience in a number of laboratories, have shown that the one uncertain step in the staining method is that of differentiation. It has been generally known that alcohols from different sources do not act alike when used for differentiation. The result of an attempt to determine the variable ingredient in the commercial 95 per cent, alcohol has yielded a method for differentiation which has given uniform results over a period of one year with a large
WOLBACH SB. THE USE OF COLOPHONIUM IN DIFFERENTIATING THE EOSIN-METHYLENEBLUE AND OTHER STAINS. JAMA. 1911;LVI(5):345–346. doi:10.1001/jama.1911.02560050031010
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