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February 12, 1916


Author Affiliations

Warren, Pa.

JAMA. 1916;LXVI(7):507. doi:10.1001/jama.1916.25810330003015b

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The simple device here illustrated has been used by me for some time. The tubes are easily prepared, can be shipped through the mails and the medium lasts indefinitely.

An ordinary 10 or 25 c.c. vaccine ampule is half filled with the culture medium—bile, bouillon, etc. The neck is then drawn to a capillary tube, and by means of an air pump connected to the water line, a vacuum of 15 or 20 inches is obtained. The capillary tube is then sealed in the flame. A rubber tube with needle is attached, and this is covered with an outer glass protecting tube as in the Keidel blood collecting tube. The whole is then sterilized intermittently or in the autoclave. The moisture in the outer, protecting tube rapidly evaporates if the tube is inverted in a rack and placed in the incubator.

For use the needle is inserted into a vein

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