Many times the laboratory worker is requested to test the virulence of diphtheria bacilli, especially in cases running for a long time, or in those occurring in institutions, and as we have had many interesting results from these tests we present some of them for consideration.
Our laboratory has always made virulence tests, on request either from the Board of Health or from the attending physician, the procedure being to isolate either by the streak-out method or surface seeding. For the former a series of eight or ten tubes is used, a fresh tube being inoculated from the incubated one; then, after sterilizing the needle, another from the freshly inoculated one, and so on through the series, taking from the first tube to the second, from the second to the third, etc., sterilizing the needle after inoculating each tube. In that way isolated colonies may be found on the later
ARMS BL, WADE EM. TESTS OF THE VIRULENCE OF DIPHTHERIA BACILLI. JAMA. 1911;LVI(11):809–810. doi:10.1001/jama.1911.02560110031013
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