In July, 1915, Gautier1 reported thirty cases of typhoid fever which had been diagnosed by the aid of the sand tube method initiated by Carnot and Hallé.
During the past three months, I have been using this method in isolating the typhoid bacilli from duodenal contents, blood, spinal fluid and feces, and because of its satisfactory results and simplicity in technic, feel that it should be called to more general notice.
The technic employed is the same as given by Gautier except that neutral red was not used:
A pipet 33 cm. long and 5 or 6 mm. in diameter is bent in a U shape. One arm is filled to the height of 10 cm. with very fine sand (passed through a No. 40 sieve). The other arm is filled with hot bouillon (tinted with neutral red.) This works up to the same level in both arms. The
M. D. Levy. THE USE OF THE SAND TUBE IN ISOLATING THE BACILLUS TYPHOSUS. JAMA. 1916;LXVI(14):1022–1023. doi:10.1001/jama.1916.25810400001015a