For over four years I have used with great satisfaction the following method for the staining of bacteria and body fluids in place of Löffler's methylen-blue, in the staining of preparations for the opsonic count instead of Leishman's or Wright's blood-stain, and in the staining of capsulated bacteria. I describe it here in the belief that it may be of help to clinicians as well as to laboratory workers.
PREPARATION OF STAINING SOLUTIONS
The preparation of this stain requires no saturated alcoholic solutions, nor the troublesome "1 to 10,000" aqueous solution of caustic potash necessary in the preparation of Löffler's methylen-blue solution. It is easily prepared in a few minutes by any physician or druggist and keeps indefinitely. The addition of a few crystals of sodium carbonate may be necessary from time to time to counteract the acidity of the corrosive sublimate which is used, as a fixative.
MEDALIA LS. A SIMPLIFIED METHOD OF STAINING BACTERIA, CAPSULATED BACTERIA IN BODY FLUIDS, AND PREPARATIONS FOR OPSONIC COUNTS. JAMA. 1911;LVI(16):1189–1190. doi:10.1001/jama.1911.02560160031009
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