The method to be described is an adaptation, on a very small scale, of the one devised by Lewis and Benedict1 for estimating the sugar-content in the blood. It is based on the fact observed by Dehn and Hartmann,2 also by Lewis and Benedict,1 that picric acid and sugar in the presence of an alkali (sodium carbonate) give, on heating, a color reaction which is proportionate to the amount of sugar present.
I have used the method of Lewis and Benedict in about three hundred tests for blood-sugar and found it admirable both in point of simplicity and accuracy.3 It entails, however, the use of 2 c.c. of blood and the employment of costly colorimetric apparatus. To overcome these drawbacks and to render the estimation of blood-sugar more suitable for general clinical and laboratory work, I have adopted the Sahli-Gower hemoglobin colorimeter (with suitable color standards)
Epstein AA. AN ACCURATE MICROCHEMICAL METHOD OF ESTIMATING SUGAR IN THE BLOOD. JAMA. 1914;LXIII(19):1667–1668. doi:10.1001/jama.1914.25710190002019b
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