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The conclusion is drawn from a considerable number of chemical analyses that the addition of 1 c.c. per 8 ounces will preserve the content of dissolved oxygen in both unpolluted and polluted water for sufficient length of time for samples to be transported to the laboratory for examination. The percentage reduction of dissolved oxygen in stored samples is believed to constitute a "fair index of appreciable pollution."
The Influence of Storage and Various Preservatives on the Dissolved Oxygen in Waters. JAMA. 1911;LVI(19):1417. doi:10.1001/jama.1911.02560190045030
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