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In this country the most popular blood stain is Wright's modification of the Romanowski method; in England and the English colonies, Leishman's stain is used almost exclusively, while in Germany, Giemsa's is the favorite. These stains and their many modifications are all excellent for chromatin, but as ordinarily used they fail to bring out the cytoplasm of the malarial parasite with the sharpness and distinctness obtained with Manson's borax methylene blue. The latter, until the Romanowski was simplified by Leishman, Wright and others, was the best we possessed for malarial parasites; in fact, for routine work it is still used by many.
The advantages of Manson's stain may be briefly stated as follows: 1. Cheapness, permanence and simplicity of preparation. It is made by dissolving 5 gm. of borax in 100 c.c. of boiling water and adding 2 gm. of methylene blue, medicinally pure; when cool the volume is made
Russell FF. A COMBINED STAINING METHOD FOR MALARIAL PARASITES AND BLOOD SMEARS. JAMA. 1915;LXIV(26):2131–2132. doi:10.1001/jama.1915.25710520003007c
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