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Article
September 8, 1917

A SIMPLE METHOD OF AGGLUTINATION OF MENINGOCOCCI

Author Affiliations

CHICAGO

From the Memorial Institute for Infectious Diseases.

JAMA. 1917;LXIX(10):786. doi:10.1001/jama.1917.02590370022009

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Abstract

The following is a simple and rapid method for differentiating by agglutination the meningococcus from other gram-negative cocci grown from the nasopharynx of meningitis patients and contacts:

Equal parts of horse serum, normal or antimeningitic, whole human blood in sodium citrate solution (1 part to 2 parts of 2 per cent. sodium citrate in salt solution), and a suspension of organisms are incubated ten minutes, smeared on a glass slide, stained and examined microscopically. In the mixture with normal horse serum there is, as a rule, very little or no clumping of the meningococcus, while in the one containing antimeningitic serum there is decided agglutination. In the event that cultures of organisms, meningococci or otherwise, should be agglutinated in mixtures with normal horse serum, it would be necessary to use dilutions of the serum, normal as well as immune, in order to bring out the specific agglutinins of the immune

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