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The need of a good diluting fluid for the purpose of counting blood corpuscles has often been impressed on me while working in the laboratory and in hospitals. Those mentioned in textbooks have many drawbacks. Some form a precipitate on standing; others while remaining clear do not permit of the addition of staining solutions so that the white corpuscles may be differentiated from the red corpuscles while making the count; still others destroy the red corpuscles if the diluted blood is permitted to remain in the counting pipet for some hours.
With the object in view of securing a fluid which will do away with the objections mentioned, I have done some work along this line and have obtained what I believe to be an ideal diluting fluid. This fluid permits of the simultaneous counting of white and red corpuscles; it keeps indefinitely without precipitating; it retains the normal shape
Jacob Diner. DILUTING FLUID FOR COUNTING BLOOD CORPUSCLES. JAMA. 1917;LXIX(17):1421. doi:10.1001/jama.1917.25910440002010c