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July 18, 1885


JAMA. 1885;V(3):81-82. doi:10.1001/jama.1885.02391030025007

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Microscopical Notes—Imbedding. 

Imbedding in Paraffin.  The object may be hardened in any fluid medium, alcohol, Müller's fluid, osmic acid or corrosive sublimate. Here it should remain for 24 to 48 hours. Then remove to chloroform, oil of bergamot, oil of turpentine or oil of cloves. Allow the object to remain here from one to 24 hours, until it is saturated. Then remove to sc paraffin (a little turpentine or bergamot, or mediur. in which the object has been soaking, should be mixed with the paraffin, to make it softer and to keep it fluid at a low temperature). Place the vessel with the parafine and object in an oven at as low temperature as it will remain fluid. Allow the object to remain in the fluid paraffin from one to 24 hours. Then place it in melted hard paraffin; let it cool after fixing

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