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The membrana limitans is a very fine, thin connective tissue layer on which the endothelia rest. It if a finely granular, or better, a finely fibrillar or striped membrane. It contains no cells. It has a watery or glass-like transparency, which is plainly observed when the endothelia are fallen off or very lightly brushed off. As Bizzozero and Salvioli have shown, the membrane is not exactly alike over all the parts of the peritoneum, but is perforated over the diaphragm. They say that the perforations of the membrana limitans are situated on the zona tendinea, and the zona peritendinea of the diaphragm. Bizzozero and Salvioli assert that the pores of the membrana limitans have a diameter from four to sixteen mm. and a round or oval circumference. They occur in groups of fifty to sixty, of irregular egg-shaped form; the pores correspond to the meshes of the fibrous connective tissue.
ROBINSON B. THE DIAPHRAGM AND CENTRUM TENDINEUM. WITH TEN ORIGINAL ILLUSTRATIONS BY THE AUTHOR. JAMA. 1897;XXVIII(6):261–266. doi:10.1001/jama.1897.02440060021001h
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