This report is a summary of our results in classifying pneumococci according to their biologic differences. The material was obtained from patients admitted to the Allegheny General Hospital and from patients in Pittsburgh and its suburbs. The technic used was identical with that developed at the Rockefeller Institute and reported by Dochez and Gillespie,1 Cole,2 Avery3 and others. The pneumococci were isolated from the sputum. In many instances, blood cultures were also taken and, when other conditions were present or developed later, such as meningitis, empyema, lung abscess, acute otitis media and mastoiditis, cultures were taken from them. In several necropsies in which pneumonia was encountered, cultures were taken from the lung, the heart's blood and the obvious accompanying lesions. The purpose of these various cultures was to determine whether the cultures of pneumococci from various lesions in the body corresponded in regard to their biologic classification.
HARTMAN CC, LACY GR. THE BIOLOGIC CLASSIFICATION IN PNEUMOCOCCIC INFECTIONS. JAMA. 1917;LXIX(26):2165–2166. doi:10.1001/jama.1917.02590530007002
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