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The authors advocate the addition of 10 c.c. of chloroform to 4 liters of sewage, followed by storage at 10° C., as the most effective method yet devised for checking the chemical changes which take place after collecting a sample of sewage. Cold storage alone is shown to be reasonably good, but higher temperatures, even when chloroform is used, are ineffective.
The Preservation of Sewage by the aid of Chloroform and Cold Storage. JAMA. 1911;LVI(21):1599. doi:10.1001/jama.1911.02560210071046
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