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Numerous modifications of the Romanowsky stain have been used in microbiology and hematology. Of these the Giemsa stain is useful in the staining of smear preparations for spirochetes and protozoa, while the Leishman, and Wright's modification of the Leishman are of greater value for the staining of blood.
In the stain described here, the method of ripening the methylene blue used by Novy and the method of obtaining solution of the dyes used by Giemsa are made use of. It has the advantage over Novy's method that a single permanent solution is used, and over Giemsa's method that the stain is of greater intensity and much less expensive.
THE ROMANOWSKY STAIN
A solution of eosin and methylene blue, made by adding an. aqueous solution of eosin to a freshly prepared aqueous solution of methylene blue, stains nuclei blue and cytoplasm red. However, when the solution of eosin is added in
McJunkin FA. A POLYCHROME STAIN WITH ADVANTAGES OVER THE GIEMSA. JAMA. 1915;LXV(25):2164–2165. doi:10.1001/jama.1915.25810250001013a
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