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For several years, in the laboratory of the Pasteur Institute of Virginia, a modification of Van Gieson's well-known method of staining for the diagnosis of rabies has been practiced. It consists essentially in the use of acetone as a solvent for rosanilin in the place of alcohol. When the solution of rosanilin in acetone is diluted with water, the stain yields itself so readily to the affinity of the cell inclusions that heat is unnecessary, as when alcohol is employed, and the inclusions are quickly tinted a brilliant rose-red color.
Furthermore, the stain may be very quickly prepared, should no fresh solution be at hand, rosanilin being extremely soluble in acetone. Nor is it necessary to wait till the solution becomes saturated, though it is preferable to keep such a solution on hand if the stain is used often. The concentrated solution is more stable than that in alcohol, and
MOON SB. MICROSCOPIC DIAGNOSIS OF RABIES: A MODIFICATION OF VAN GIESON'S METHOD FOR STAINING THE CHARACTERISTIC NERVE CELL INCLUSIONS. JAMA. 1911;LVII(9):735. doi:10.1001/jama.1911.04260080299018
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