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November 11, 1911


Author Affiliations


From the Laboratories of the Rockefeller Institute for Medical Research.

JAMA. 1911;LVII(20):1611. doi:10.1001/jama.1911.04260110111014

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The duration of the life of cultures of tissues, up to now, has been very brief. In approximately from three to fifteen days after the preparation of the culture, the growth becomes progressively less rapid until it stops altogether. Following this; the tissues die and the cells disintegrate.

It may easily be supposed that senility and death of tissues are not a necessary phenomenon and that they result merely from accidental causes, such as accumulation of catabolic substances and exhaustion of the medium. The suppression, then, of these causes should bring about the rejuvenation of the arrested culture and thus increase considerably the duration of its life. As it would be important, for many reasons, to keep tissues alive outside of the organism for a long period of time, I attempted to develop a method for the rejuvenation of the cultures of tissues.

The rejuvenation consists in removing from the

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