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March 2, 1912


JAMA. 1912;LVIII(9):632. doi:10.1001/jama.1912.04260030032012

Recently J. H. Schrup of Dubuque, Ia., described the use of the vacuum-bottle as a means of incubating bacterial cultures. As I have had some experience with the method I think that it may not be amiss, while interest in the subject is still fresh, to communicate a brief summary of the observations which I have made.

The vacuum-bottle occurred to me as a convenient expedient in a case of some urgency seen last June in the country where no laboratory method of incubation was available. I had never heard of a vacuum-bottle being used for this purpose before, and, after several satisfactory trials, it seemed so valuable a resource that I undertook some experiments to test its reliability under varying conditions. One series of experiments was made in June, 1911, during very hot weather, and I had intended, for comparison, to make a second series of tests during