For those who desire to make routine observations on the presence of peptid hydrolyzing enzymes in stomach extracts (particularly in cases of carcinoma ventriculi), the procedure here described may prove of value. It has been employed in the laboratory of gastro-enterology at St. Mary's Hospital (Mayo Clinic) in more than 800 cases. The results have been as dependable as with the original "glycyltryptophan test" described by Neubauer and Fischer.1
The preparation of this dipeptid, regularly sent out by Kalle and Company (Biebrich am Rhein), under the direction of Neubauer and Fischer, consists of practically 1 c.c. of a glycyltryptophan solution, under about two times its volume of toluol. This is contained in a thick-walled, glass-stoppered bottle. The body of the bottle is marked, just below its neck, to indicate to what extent the volume of contained glycyltryptophan solution is to be diluted with filtered gastric extract. Examination
SMITHIES F. A SIMPLIFIED TECHNIC FOR THE APPLICATION OF THE GLYCYLTRYPTOPHAN TEST TO GASTRIC CONTENTS. JAMA. 1912;LVIII(14):1008–1009. doi:10.1001/jama.1912.04260040024011
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