While it has been generally conceded for some years that the mere fact of freezing did not kill bacteria, but only rendered them for the time incapable of multiplication, there has been a very general impression that the keeping of bacteria at a temperature below the freezing point for a long period eventually led to their death. Such intensely low temperatures as may be produced by liquid air fail to destroy at least the more resistant forms of bacteria. On the other hand, Sedgwick, Winslow and Park in the course of their investigation of the ice supply of Boston came to the conclusion that ice, even from contaminated water, became sterile after a month or perhaps even less. In a review of their work in the Boston Medical and Surgical Journal1 it is stated that it may be concluded from these investigations that ice more than three weeks old
PROLONGED FREEZING AND GERM LIFE.. JAMA. 1903;XLI(15):913–914. doi:10.1001/jama.1903.02490340021007
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